| Team | Constructor | Motorcycle | No. | Riders | ||
|---|---|---|---|---|---|---|
 | Aprilla Racing | Aprilla | RS-GP | 12 41 |  | Maverick Vinales Aleix Espargaro |
 | CryptoData Aprilla RNF MotoGP Team | Aprilla | RS-GP | 25 88 |  | Raul Fernandez Miguel Oliveira |
| Ducati Lenovo Team | Ducati | Desmosedici GP23 | 1 23 | | Francesco Bagnaia Enea Bastianini |
| Prima Pramac Racing | Ducati | Desmosedici GP23 | 5 89 |  | Johann Zarco Jorge Martin |
| Gresini Racing MotoGP | Ducati | Desmosedici GP22 | 49 73 | | Fabio Di Giannantonio Alex Marquez |
| Mooney VR46 Racing Team | Ducati | Desmosedici GP22 | 10 72 | | Luca Marini Marco Bezzecchi |
| Tech3 GasGas Factory Racing | GasGas | RC16 | 37 44 | | Augusto Fernandez Pol Espargaro |
 | LCR Honda Idemitsu LCR Honda Castrol | Honda Honda | RC213V RC213V | 30 42 |  | Takaaki Nakagami Alex Rins |
| Repsol Honda Team | Honda | RC213V | 36 93 | | Joan Mir Marc Marquez |
 | Red Bull KTM Factory Racing | KTM | RC16 | 33 43 |   | Brad Binder Jack Miller |
| Monster Energy Yamaha MotoGP Team | Yamaha | YZR-M1 | 20 21 | | Fabio Quartararo Franco Morbidelli |
2023 MotoGP Team/Rider Line-Up
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